LOCUS       sequence-153980-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  QF2 HACK backbone compatible with GMR attP integrated insertions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Lin CC, Potter CJ
  TITLE     Editing Transgenic DNA Components by Inducible Gene Replacement in 
            Drosophila melanogaster.
  JOURNAL   Genetics. 2016 Jun 22. pii: genetics.116.191783.
  PUBMED    27334272
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Jun 22. pii: genetics.116.191783."
COMMENT     Sequence Label: pBPGUw-HACK-QF2
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        184..259
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        260..680
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        699..774
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aagggattcc tatccggcgg tagtcccatc attggcatgg taggtacccg aaagcaaccc
       61 cttagttaca gacacagcgc gtacgtcctt cgcatcctta tgattcccaa gtacatattc
      121 tgcaagagta cagtatatat aggaaagata tccgggtgaa cttcggggtc ttcgagaaga
      181 cctgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
      241 agtggcaccg agtcggtgct tttttgctca cctgtgattg ctcctactca aatacaaaaa
      301 catcaaattt tctgtcaata aagcatattt atttatattt attttacagg aaagaattcc
      361 ttttaaagtg tattttaacc tataatgaaa aacgattaaa aaaaatacat aaaataattc
      421 gaaaattttt gaatagccca ggttgataaa aattcatttc atacgtttta taacttatgc
      481 ccctaagtat tttttgacca tagtgtttca attctacatt aattttacag agtagaatga
      541 aacgccacct actcagccaa gaggcgaaaa ggttagctcg ccaagcagag agggcgccag
      601 tgctcactac tttttataat tctcaacttc tttttccaga ctcagttcgt atatatagac
      661 ctattttcaa tttaacgtcg gggtcttcga gaagacctgt tttagagcta gaaatagcaa
      721 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
      781 gcctacctgg agcctgagag ttgttcaata aaataaaaat gtttcgtttt tttgctttcg
      841 ccagtattta ttatttttca tcaatatgta ttcaatttgg tatgt
//
