LOCUS       sequence-153989-         296 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid, non-targeting control gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: non-targeting control gRNA (BRDN0001147195)
FEATURES             Location/Qualifiers
     source          1..296
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        158..233
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
       61 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
      121 gaaaggacga aacaccgtcg ggcagtgagt acaatacgtt ttagagctag aaatagcaag
      181 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctttttta
      241 agcttggcgt aactagatct tgagacaaat ggcagtattc atccacaatt ttaaaa
//
