LOCUS       sequence-153991-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of genes (PGK 
            promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim 
            WA
  TITLE     Engineering Customized Cell Sensing and Response Behaviors Using 
            Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. 
            Epub 2016 Jan 28.
  PUBMED    26830878
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.01.012"; journalName: "Cell"; date: 
            "2016-02-11- 11"; volume: "164"; issue: "4"; pages: "780-91"
COMMENT     Sequence Label: pHR_PGK
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        74..573
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(109..132)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     506..525
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(776..796)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg atcgataagc
       61 ttgatatcga attgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
      121 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
      181 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
      241 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
      301 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
      361 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
      421 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
      481 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
      541 ctgttctcct cttcctcatc tccgggcctt tcgaattctc acgcgtcaag tggagcaagg
      601 caggtggaca gtggatcctt gacttgcggc cgcaactccc acctgcaaca tgcgtgactg
      661 actgaggccg cgactctaga gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg
      721 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
      781 ctccttttac gctatgtgga tacgctgct
//
