LOCUS       sequence-153995-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of genes with Gal4DBDVP64
            synNotch Receptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim 
            WA
  TITLE     Engineering Customized Cell Sensing and Response Behaviors Using 
            Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. 
            Epub 2016 Jan 28.
  PUBMED    26830878
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.01.012"; journalName: "Cell"; date: 
            "2016-02-11- 11"; volume: "164"; issue: "4"; pages: "780-91"
COMMENT     Sequence Label: pHR_Gal4UAS_IRES_mC_pGK_tBFP
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     240..264
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(526..543)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     753..772
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg atcgataagc
       61 ttgatatcga attcggagca ctgtcctccg aacgtcggag cactgtcctc cgaacgtcgg
      121 agcactgtcc tccgaacgtc ggagcactgt cctccgaacg gagcatgtcc tccgaacgtc
      181 ggagcactgt cctccgaacg actagttagg cgtgtacggt gggaggccta tataagcaga
      241 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      301 agaagacacc gggaccgatc cagcctctcg acattcgttg gatccttgac ttgcggcccc
      361 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
      421 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
      481 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
      541 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
      601 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
      661 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
      721 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
      781 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
      841 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggcccc
//
