LOCUS       sequence-153996-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of genes with Gal4DBDVP64
            synNotch Receptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim 
            WA
  TITLE     Engineering Customized Cell Sensing and Response Behaviors Using 
            Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. 
            Epub 2016 Jan 28.
  PUBMED    26830878
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.01.012"; journalName: "Cell"; date: 
            "2016-02-11- 11"; volume: "164"; issue: "4"; pages: "780-91"
COMMENT     Sequence Label: pHR_Gal4UAS_IRES_mC_pGK_tBFP
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(60..761)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(830..849)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcaagcttgc atgcctgcat
       61 taattaagct tgtgccccag tttgctaggg aggtcgcagt atctggccac tgccacctcg
      121 tgctgctcga cgtaggtctc gttgttggcc tccttgattc tttccagtct gtagtccaca
      181 tagtagacgc caggcatctt gaggttctta gcgggtttct tggatctata tgtggtcttg
      241 atgtttgcga tcagatggct cccgcccacg agcttcaggg ccatgtcgtt tctgccttcc
      301 aggccgccgt cagcggggta cagcgtctcg gtgaaggcct cccagccgag tgttttcttc
      361 tgcatcacag ggccgttgga tgtgaagttc acccctctga tcttgacgtt gtagatgagg
      421 cagccgtcct ggaggctggt gtcctgggta gcggtcagca cgcccccgtc ttcgtatgtg
      481 gtgactctct cccatgtgaa gccctcaggg aaggactgct tgaagaagtc ggggatgccc
      541 tgggtgtggt tgatgaaggt cttgctgccg tagaggaagc tagtagccag gatgtcgaag
      601 gcgaagggga gagggccgcc ctcgaccacc ttgattctca tggtctgggt gccctcgtag
      661 ggcttgcctt cgccctcgga tgtgcacttg aagtgatggt tgtccacggt gccctccatg
      721 tacagcttca tgtgcatgtt ctccttaatc agctcgctca tggtaagctt gggctgcagg
      781 tcgaaaggcc cggagatgag gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg
      841 tgcagaatgc cgggcctcc
//
