LOCUS       sequence-154011-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional interference assessment; strong interfering 
            promoter, 500 bp spacer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Hoffmann SA, Kruse SM, Arndt KM
  TITLE     Long-range transcriptional interference in E. coli used to construct
            a dual positive selection system for genetic switches.
  JOURNAL   Nucleic Acids Res. 2016 Jun 2;44(10):e95. doi: 10.1093/nar/gkw125. 
            Epub 2016 Feb 29.
  PUBMED    26932362
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-06-2"; volume: "44(10):e95. doi"; pages: " 
            10.1093/nar/gkw125. Epub 2016 Feb 29"
COMMENT     Sequence Label: SAH0146-0.5-conP01
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(306..325)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(1030..1049)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacatc aagaccacat
       61 atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat gtggactaca
      121 gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac gaggtggcag
      181 tggccagata ctgcgacctc cctagcaaac tggggcacaa gctaaattaa aagctaaccg
      241 gggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
      301 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
      361 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
      421 acattcttgc ccgcctgatg aatgctcatc cggagttccg tatggcaatg aaagacggtg
      481 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
      541 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
      601 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
      661 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
      721 ccaatatgga caacttaccg gtcagaagct tttacttgta cagctcgtcc atgccgccgg
      781 tggagtggcg gccctcggcg cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg
      841 aggtgatgtc caacttgatg ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg
      901 ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct
      961 gcttgatctc gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc
     1021 agcccatggt cttcttctgc attacggggc c
//
