LOCUS       sequence-154012-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional interference assessment; strong interfering 
            promoter, 500 bp spacer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Hoffmann SA, Kruse SM, Arndt KM
  TITLE     Long-range transcriptional interference in E. coli used to construct
            a dual positive selection system for genetic switches.
  JOURNAL   Nucleic Acids Res. 2016 Jun 2;44(10):e95. doi: 10.1093/nar/gkw125. 
            Epub 2016 Feb 29.
  PUBMED    26932362
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-06-2"; volume: "44(10):e95. doi"; pages: " 
            10.1093/nar/gkw125. Epub 2016 Feb 29"
COMMENT     Sequence Label: SAH0146-0.5-conP01
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(43..49,50..65,66..71))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     terminator      126..155
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      311..405
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 tcgctcatct agtaggtttc ctgtgtgact ctagattcca cacattatac gagccgatga
       61 ttaattgtca agagaattcg agtcactaag ggctaactaa ctaattacgt agcaatcaac
      121 tcactggctc accttcacgg gtgggccttt cttcggcacg ggcaaattgc tgaatattcc
      181 ttttcttaga cgtcaggtgg caaacgcgaa gtaatctttt cggttttaaa gaaaaagggc
      241 agggtggtga caccttgccc gtttttttgc cggagctaga gctgtcagac caagtttacg
      301 agctcgcttg gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg
      361 ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag cactagggac
      421 agtaagacgg gtaagcctgt tgatgatacc gctgccttac tgggtgcatt agccagtctg
      481 aatgacctgt cacgggataa tccgaagtgg tcagactgga aaatcagagg gcaggaactg
      541 ctgaacagca aaaagtcaga tagcaccaca tagcagaccc gccataaaac gccctgagaa
      601 gcccgtgacg ggcttttctt gtattatggg tagtttcctt gcatgaatcc ataaaaggcg
      661 cctgtagtgc catttacccc cattcactgc cagagccgtg agcgcagcga actgaatgtc
      721 acgaaaaaga cagcgactca ggtgcctgat ggtcggagac aaaaggaata ttcagcgatt
      781 tgcccgagct tgcgagggtg ctacttaagc ctttagggtt ttaaggtctg ttttgtagag
      841 gagcaaacag cgtttgcgac atccttttgt aatactgcgg aactgactaa agtagtgagt
      901 tatacacagg gctgggatct attcttttta tctttttt
//
