LOCUS       sequence-154019-        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRSETa (Invitrogen) plasmid expressing Amrose variant 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Schoetz U, Deliolanis NC, Ng D, Pauli J, Resch-Genger U, Kuhn E, 
            Heuer S, Beisker W, Koster RW, Zitzelsberger H, Caldwell RB
  TITLE     Usefulness of a Darwinian system in a biotechnological application: 
            evolution of optical window fluorescent protein variants under 
            selective pressure.
  JOURNAL   PLoS One. 2014 Sep 5;9(9):e107069. doi: 
            10.1371/journal.pone.0107069. eCollection 2014.
  PUBMED    25192257
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-09-5"; volume: "9(9):e107069. doi"; pages: " 
            10.1371/journal.pone.0107069. eCollection 2014"
COMMENT     Sequence Label: pRSETa-Amrose v1
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(50..505)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(187..208)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     399..418
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(602..649)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     645..663
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
       61 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
      121 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
      181 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
      241 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
      301 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
      361 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
      421 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
      481 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
      541 gggcgatcgg tgcgggcctc ttcgctatta cgccagatcc ggatatagtt cctcctttca
      601 gcaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctagt tattgctcag
      661 cggtggcagc agccaactca gcttcctttc gggctttgtt agcagccgga tcaagcttcg
      721 aattccatgg taccagctgc agatctcgag ctcgagtcat ctgtgcccca gtttgctagg
      781 gaggtcgcag tacttggcca cagccatctc gtgctgctcg acgtaggtct ctttgtcggc
      841 ctccttgatt ctttccagtc tgtagtccac gaagtggaag ccgggcatct tgaggttctt
      901 agcgggtttc ttggatctgt atgtggtctt gagggagcag tgcaggtagc ccccgcccac
      961 gagcttcagg gccatctggt tatggcctct caggccgctg tcagcggggt acatcatctc
     1021 ggtgctggcc tcccagccga gtgttttctt ctgcatcaca gggccgttgg atgg
//
