LOCUS       sequence-154021-        1085 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRSETa (Invitrogen) plasmid expressing Amrose variant 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Schoetz U, Deliolanis NC, Ng D, Pauli J, Resch-Genger U, Kuhn E, 
            Heuer S, Beisker W, Koster RW, Zitzelsberger H, Caldwell RB
  TITLE     Usefulness of a Darwinian system in a biotechnological application: 
            evolution of optical window fluorescent protein variants under 
            selective pressure.
  JOURNAL   PLoS One. 2014 Sep 5;9(9):e107069. doi: 
            10.1371/journal.pone.0107069. eCollection 2014.
  PUBMED    25192257
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-09-5"; volume: "9(9):e107069. doi"; pages: " 
            10.1371/journal.pone.0107069. eCollection 2014"
COMMENT     Sequence Label: pRSETa-Amrose v2
FEATURES             Location/Qualifiers
     source          1..1085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(51..506)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(188..209)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     400..419
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(603..650)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     646..664
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
       61 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
      121 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
      181 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
      241 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
      301 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
      361 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
      421 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
      481 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
      541 agggcgatcg gtgcgggcct cttcgctatt acgccagatc cggatatagt tcctcctttc
      601 agcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag ttattgctca
      661 gcggtggcag cagccaactc agcttccttt cgggctttgt tagcagccgg atcaagcttc
      721 gaattccatg gtaccagctg cagatctcga gctcgagtca tctgtgcccc agtttgctag
      781 ggaggtcgca gtacttggcc acagccatct cgtgctgctc gacgtaggtc tctttgtcgg
      841 cctccttgat tctttccagt ctgtagtcca cgaagtggaa gccgggcatc ttgaggttgt
      901 tagcgggttt cttggatctg tatgtggtct tgagggagca gtgcaggtag cccccgccca
      961 cgagcttcag ggccatctgg ttatggcctc tcaggccgct gtcagcgggg tacatcatct
     1021 cggtgctggc ctcccagccg agtgttttct tctgcatcac agggccgttg gatgggaagt
     1081 ccacc
//
