LOCUS       sequence-154022-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRSETa (Invitrogen) plasmid expressing Amrose variant 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Schoetz U, Deliolanis NC, Ng D, Pauli J, Resch-Genger U, Kuhn E, 
            Heuer S, Beisker W, Koster RW, Zitzelsberger H, Caldwell RB
  TITLE     Usefulness of a Darwinian system in a biotechnological application: 
            evolution of optical window fluorescent protein variants under 
            selective pressure.
  JOURNAL   PLoS One. 2014 Sep 5;9(9):e107069. doi: 
            10.1371/journal.pone.0107069. eCollection 2014.
  PUBMED    25192257
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-09-5"; volume: "9(9):e107069. doi"; pages: " 
            10.1371/journal.pone.0107069. eCollection 2014"
COMMENT     Sequence Label: pRSETa-Amrose v2
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     318..337
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        318..336
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             368..390
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             410..427
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             431..463
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(467..475,476..490)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      241 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagga
      301 tctcgatccc gcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag
      361 aaataatttt gtttaacttt aagaaggaga tatacatatg cggggttctc atcatcatca
      421 tcatcatggt atggctagca tgactggtgg acagcaaatg ggtcgggatc tgtacgacga
      481 tgacgataag gatcgatggg gatccatgag cgagctgatc aaggagaaca tgcacatgaa
      541 actgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg
      601 caagccctac gagggcaccc agaccatgaa gatcaaggtg gtcgagggcg gccctctccc
      661 cttcgccttc gacatcctgg ctaccagctt catgtacggc agcaaagcct tcatcaacca
      721 cacccagggc atccccgact tctttaagga gtccttccct gagggcttca catgggagag
      781 aatcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagaa
      841 cggctgcctc atctacaacg tcaagatcaa cggggtggac ttcccatcca acggccctgt
      901 gatgcagaag aaaacactcg gctgggaggc cagcaccgag atgatgtacc ccgctgacag
      961 cggcctgaga ggccataacc agatggccct gaagctcgtg ggcgggggct acctgcactg
     1021 ctccctcaag accacataca gatcca
//
