LOCUS       sequence-154029-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TRAP 4 fused to mEOS3.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  TITLE     FtsZ-MEEVF
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TRAP 4-mEOS
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(90..108,109..114,115..131,132..137,138..163)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    90..108
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    115..133
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             173..184
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
       61 tgagttttcg ttccactgag cgaagatctt ccctatcagt gatagagatt gacatcccta
      121 tcagtgatag agatactgag cacatcagca ggacgcactg accgaattca ttaaagagga
      181 gaaaggtacc atgaagcagg cactgaaaga aaaagagctg gggaacgatg cctacaagaa
      241 gaaagacttt gacacagcct tgaagcatta cgacaaagcc aaggagctgg accccactaa
      301 catgacttac attatcaatc aagcagcggt atactttgaa aagggcgact acaataagtg
      361 ccgggagctt tgtgagaagg ccattgaagt ggggagagaa aaccgagagg actatcgatg
      421 gattgccatt gcatatgctc gaattggcaa ctcctacttc aaagaagaaa agtacaagga
      481 tgccatccat ttctataaca agtctctggc agagcaccga accccaaagg tgctaaaaaa
      541 gtgccaacag gcggagaaaa tcctgaagga gcaaggcggc agcggcagcg gcctgcagat
      601 gagtgcgatt aagccagaca tgaagatcaa actccgtatg gaaggcaacg taaacgggca
      661 ccactttgtg atcgacggag atggtacagg caagcctttt gagggaaaac agagtatgga
      721 tcttgaagtc aaagagggcg gacctctgcc ttttgccttt gatatcctga ccactgcatt
      781 ccattacggc aacagggtat tcgccaaata tccagacaac atac
//
