LOCUS       sequence-154032-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FtsZ fused to the MEEVF peptide tag via a flexible linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  TITLE     FtsZ-MEEVF
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FtsZ-MEEVF
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..27
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11..27
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(43..60)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(43..59)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(52..74)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     217..235
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(259..273,274..279,280..296,297..303)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    279..295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    311..327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             346..357
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 acatttcaca caggaaacag ctatgaccat gattacggat tcactggccg tcgttttaca
       61 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      121 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcc
      181 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
      241 cgtcttcacc tcgagaaatc ataaaaaatt tatttgcttt gtgagcggat aacaattata
      301 atagattcaa ttgtgagcgg ataacaattt cacacagaat tcattaaaga ggagaaatta
      361 actactagta tgtttgaacc aatggaactt accaatgacg cggtgattaa agtcatcggc
      421 gtcggcggcg gcggcggtaa tgctgttgaa cacatggtgc gcgagcgcat tgaaggtgtt
      481 gaattcttcg cggtaaatac cgatgcacaa gcgctgcgta aaacagcggt tggacagacg
      541 attcaaatcg gtagcggtat caccaaagga ctgggcgctg gcgctaatcc agaagttggc
      601 cgcaatgcgg ctgatgagga tcgcgatgca ttgcgtgcgg cgctggaagg tgcagacatg
      661 gtctttattg ctgcgggtat gggtggtggt accggtacag gtgcagcacc agtcgtcgct
      721 gaagtggcaa aagatttggg tatcctgacc gttgctgtcg tcactaagcc tttcaacttt
      781 gaaggcaaga agcgtatggc attcgcggag caggggatca ctgaactgtc c
//
