LOCUS       sequence-154034-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac backbone, CAG promoter driving murine Tfe3-ERT2 fusion, 
            Hygromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith 
            A
  TITLE     Exit from pluripotency is gated by intracellular redistribution of 
            the bHLH transcription factor Tfe3.
  JOURNAL   Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012.
  PUBMED    23582324
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.03"; journalName: "Cell"; date: "2013-04-11- 
            11"; volume: "153"; issue: "2"; pages: "335-47"
COMMENT     Sequence Label: pPB-CAG-Tfe3::ERT2-pgk-hph
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(227..260)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    337..561
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     550..567
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    627..648
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(663..668,669..686,687..693)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    701..717
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     706..728
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     725..741
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     725..741
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    762..786
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aagataatca tattgtgacg tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt
       61 ttatcggtct gtatatcgag gtttatttat taatttgaat agatattaag ttttattata
      121 tttacactta catactaata ataaattcaa caaacaattt atttatgttt atttatttat
      181 taaaaaaaaa caaaaactca aaatttcttc tataaagtaa caaaacataa cttcgtatag
      241 catacattat acgaagttat ctcgacgcgg ccgctctagc ctcgaggcta gaactagtgg
      301 atctcgagcc ccagctggtt ctttccgcct cagaagccat agagcccacc gcatccccag
      361 catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca
      421 gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga
      481 cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg
      541 gctggcaact agaaggcaca gtcgaggctg atccgctggc acgacaggtt tcccgactgg
      601 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      661 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      721 cacacaggaa acagctatga ccatgattac gccaagctat caccactttg tacaagaaag
      781 ctgggtctca tcagactgtg gcagggaaac cctctgcctc ccccgtgatg taatactttt
      841 gcaaggaatg cgatgaagta gagcccgcag tggccaagtg gctttggtcc gtctcctcca
      901 cggatgcccc tccacggcta gtgggcgcat gtagg
//
