LOCUS       sequence-154037-         569 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector containing SFFV promoter, dCas9 that is 
            fused to 2x NLS, tagBFP and a KRAB domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pHR-SFFV-dCas9-BFP-KRAB
FEATURES             Location/Qualifiers
     source          1..569
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(88..273)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcaagcttgc atgcctgcag
       61 gtcgactcta gagtcgcggc cttactaggg ctcttctccc ttctccaacc ggaggatcac
      121 atctggctta gtaagctgat aacccaagga aaccaggttc ttatagttct ccagcatcac
      181 atttctgtac acgatctgct gagcagtgtc cagcagcttc cactcctccc tggtgaagtc
      241 cacaaataca tccttgaagg tcaccagtgt ccgggaccag gcagttagtg acttagcatc
      301 catccctccg ccaccgccat taagcttgtg ccccagtttg ctagggaggt cgcagtatct
      361 ggccactgcc acctcgtgct gctcgacgta ggtctcgttg ttggcctcct tgattctttc
      421 cagtctgtag tccacatagt agacgccagg catcttgagg ttcttagcgg gtttcttgga
      481 tctatatgtg gtcttgatgt ttgcgatcag atggctcccg cccacgagct tcagggccat
      541 gtcgtttctg ccttccaggc cgccgtcag
//
