LOCUS       sequence-154038-         593 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector containing SFFV promoter, dCas9 that is 
            fused to 2x NLS, tagBFP and a KRAB domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pHR-SFFV-dCas9-BFP-KRAB
FEATURES             Location/Qualifiers
     source          1..593
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcctccgaca gactgagtcg cccggggggg atctggagct ctcgagaatt ctcacgcgtc
       61 tgcaggatat caagcttgcg gtaccgcggg cccggccacc atggacaaga agtacagcat
      121 cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt
      181 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct
      241 gatcggcgcc ctgctgttcg acagcggaga aacagccgag gccacccggc tgaagagaac
      301 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag
      361 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt
      421 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc
      481 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga
      541 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tcc
//
