LOCUS       sequence-154040-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for sgRNA and for Expression of Cas9 linked to BFP
            via T2A linked to Ad4 E1B via P2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
  TITLE     Increasing the efficiency of homology-directed repair for 
            CRISPR-Cas9-induced precise gene editing in mammalian cells.
  JOURNAL   Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
  PUBMED    25803306
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198."
COMMENT     Sequence Label: pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cccgcttgac ccgaactcag tgccagtgca ggacaggacc agatgatcag ggcgcagatc
       61 ctctgtcacg tccacgcaca cgggctgaaa ccgagcgtgc ttccctccac actcgcaggc
      121 cctgcatcga gttttatatt cgtcgtagcg caggatcttc cagacctcca cattcatatc
      181 gaacacgcct gtcagagaca cccgggacat cacgtcgggt tccagcagga ctttcacata
      241 attcaggtta cactggtatg gcataaacat gccgcgccgg gcccccagat gcatattgca
      301 tctagtcatc acgttgtgtt cgaactcggg ccatggcttt cgactatgag aagcgacgtg
      361 cactgcagcc agcatatgac tgttgccccc ggcacatgtc agcatctggt accctctctc
      421 gtcagaggct ccgcaaatca tattgtgttt gatctttgcg tttcctttgc acagcacgaa
      481 gcagccggtc tcagtggagg cacaatggcg aatccgtgct tctccctcgc tcatcacgcc
      541 caggtgacat ctttcaaaca ggcatttctt cacgctcagc attgattttg tcctcccgac
      601 cactcccatc cagtttgcgg agaagctgca gcccttgacc cccacttgtc cccaagcctc
      661 gatacaggta ttgttaaacc caaagaagga gcatccatgc acagtcagct ttgtattagc
      721 catgaagacg gtgccgttat agccatcccc tctaaacctc atattcatga aagtcacgcc
      781 atccatgtcg accaccccgg gg
//
