LOCUS       sequence-154042-         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Sakuma T, Nakade S, Sakane Y, Suzuki KT, Yamamoto T
  TITLE     MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the 
            PITCh systems.
  JOURNAL   Nat Protoc. 2016 Jan;11(1):118-33. doi: 10.1038/nprot.2015.140. Epub
            2015 Dec 17.
  PUBMED    26678082
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.140"; journalName: "Nat Protoc"; date: 
            "2016-01"; volume: "11"; issue: "1"; pages: "118-33"
COMMENT     Sequence Label: pCRIS-PITChv2-FBL
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    424..440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(648..669)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 acgagtaatc gttaatccgc aaataacgta aaaacccgct tcggcgggtt tttttatggg
       61 gagagtttag ggaaagagca tttgtcagaa tatttaaggg cgcctgtcac tttgcttgat
      121 atatgagaat tatttaacct tataaatgag aaaaaagcaa cgcactttaa ataagatacg
      181 ttgctttttc gattgatgaa cacctataat taaactattc atctattatt tatgattttt
      241 tgtatataca atatttctag tttgttaaag agaattaaga aaataaatct cgaaaataat
      301 aaagggaaaa tcagtttttg atatcaaaat tatacatgtc aacgataata caaaatataa
      361 tacaaactat aagatgttat cagtatttat tatcatttag aataaatttt gtgtcgccct
      421 taattgtgag cggataacaa ttacgagctt catgcacagt ggcgttgaca ttgattattg
      481 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
      541 cgcgttacat agcatcgtac gcgtacgtgt ttggttcctc tcacaggcca ccccccggat
      601 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      661 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgcc
//
