LOCUS       sequence-154053-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for 
            in vivo CRISPRa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Lin S, Ewen-Campen B, Ni X, Housden BE, Perrimon N
  TITLE     In Vivo Transcriptional Activation Using CRISPR/Cas9 in Drosophila.
  JOURNAL   Genetics. 2015 Oct;201(2):433-42. doi: 10.1534/genetics.115.181065. 
            Epub 2015 Aug 5.
  PUBMED    26245833
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.115.181065"; journalName: "Genetics"; date: 
            "2015-10"; volume: "201"; issue: "2"; pages: "433-42"
COMMENT     Sequence Label: pWalium20-10XUAS-3XFLAG-dCas9-VPR
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             100..165
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             172..192
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actctgaata gggaattggg aattgagatc tgttctagaa aacatcccat aaaacatccc
       61 atattcagcc gctagcatgg atgttttccc gccaccatgg attacaagga tcacgatggc
      121 gactacaagg accatgacat cgactataag gacgacgacg ataagatggc ccccaagaag
      181 aagcgcaaag tgggcatcca tggcgtgcca gccgccgaca agaagtactc cattgggctc
      241 gctatcggca caaacagcgt cggctgggcc gtcattacgg acgagtacaa ggtgccgagc
      301 aaaaaattca aagttctggg caataccgat cgccacagca taaagaagaa cctcattggc
      361 gccctcctgt tcgactccgg ggagacggcc gaagccacgc ggctcaaaag aacagcacgg
      421 cgcagatata cccgcagaaa gaatcggatc tgctacctgc aggagatctt tagtaatgag
      481 atggctaagg tggatgactc tttcttccat aggctggagg agtccttttt ggtggaggag
      541 gataaaaagc acgagcgcca cccaatcttt ggcaatatcg tggacgaggt ggcgtaccat
      601 gaaaagtacc caaccatata tcatctgagg aagaagcttg tagacagtac tgataaggct
      661 gacttgcggt tgatctatct cgcgctggcg catatgatca aatttcgggg acacttcctc
      721 atcgaggggg acctgaaccc agacaacagc gatgtcgata aactctttat ccaactggtt
      781 cagacttaca atcagctttt cgaagagaac ccgatcaacg catccggagt tgacgcc
//
