LOCUS       sequence-154054-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for 
            in vivo CRISPRa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Lin S, Ewen-Campen B, Ni X, Housden BE, Perrimon N
  TITLE     In Vivo Transcriptional Activation Using CRISPR/Cas9 in Drosophila.
  JOURNAL   Genetics. 2015 Oct;201(2):433-42. doi: 10.1534/genetics.115.181065. 
            Epub 2015 Aug 5.
  PUBMED    26245833
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.115.181065"; journalName: "Genetics"; date: 
            "2015-10"; volume: "201"; issue: "2"; pages: "433-42"
COMMENT     Sequence Label: pWalium20-10XUAS-3XFLAG-dCas9-VPR
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(114..683)
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
ORIGIN
        1 tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta tgtcacacca
       61 cagaagtaag gttccttcac aaagatcccg cggctctagt tctttgcaat tcaaaacaga
      121 gatgtgtcga agatggacag tcctgtgctg atatgcatgg catgcaagag gcactcgtcg
      181 ttcaggaagg tatccagaat ctcgttcaat tccggggtca ggggtgagtc caggttcaga
      241 tcctcggtca tggactcaag tgtggttgtc agctcatcca gatggcccct tgggggcgga
      301 tgggaaaggt ccatttggcc acagattgca gcctcttcct tctggggaat cacagtatcg
      361 gccatctccc gaagggcttt gacagcctgg ctcgtctctt catcgggatc ctccaacagg
      421 tgactggcct cgggagtcac tgcgggcgct ggatccagtg gctgagggac tggtgccggg
      481 gtcagtgacc cgactggctc atgtactgga ccggttggtg ttggtgcgag gctggcgggg
      541 agtgggcggt tggcccatgg acttcctgga ggatgaaatg gccggattcg ttttggctgg
      601 cacacctcgc ggccctcaaa cacgtcacta atagcggagc cggcctcagg cttcggcaaa
      661 aacatccctt ccctggaatc ccgagatccc gatccgctac ccagcagtgc ggagaagtcc
      721 atgtcggcga tagagctgaa gtcctcgtcg ccagacagca gtccatta
//
