LOCUS       sequence-154055-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged Dm4E-T in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Igreja C, Peter D, Weiler C, Izaurralde E
  TITLE     4E-BPs require non-canonical 4E-binding motifs and a lateral surface
            of eIF4E to repress translation.
  JOURNAL   Nat Commun. 2014 Sep 2;5:4790. doi: 10.1038/ncomms5790.
  PUBMED    25179781
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-09-2"; volume: "5:4790. doi"; pages: " 
            10.1038/ncomms5790"
COMMENT     Sequence Label: pAc5.1B-EGFP-Dm4E-T
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..24
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(47..64)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(74..115)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 aggccttaga aggcacagtc gaggctgatc agcgggttta aactcaatgg tgatggtgat
       61 gatgaccggt acgcgtagaa tcgagaccga ggagagggtt agggataggc ttaccttcga
      121 accgcgggcc ctctagactc gagcggccgc cttagttatg cacgacgcct gaggaatgtt
      181 gaatgcttcg ttcaaattct tccaagctga gtgcttggtt cacatttaaa agtggcagtt
      241 ttccagcaga ggcttttgca agaagctcag gggaaaacca ttgcgccaat tgattggttg
      301 gtgatgaaat cctctcatct ctctggtagc taggagatgt gaggctgtta gctttgatca
      361 cattaccgga atcggagctg tcagaaaaac ctgggactgg cggagacatt tgtggatttg
      421 attgacggtg catttctcca taaatcactc tttgactaag ctgctgtcgt gtgtgatgat
      481 gttgttgctg atgttgcatg taaagttggt gccatccaac aggagatgta atgttttggt
      541 gaacactttc ggtattcaga tttgattcca cagattgaac tgctttaaat ctatgttgct
      601 gctgctgtag atgtggttga tgaatgggtt gcatttcaat tttatgggca gtaaaaggaa
      661 gcgcagaatt ggaatgcggc attgaattaa gacctccttt cagaataggt cggccaaatg
      721 tttttccgtg gaccatttgc atattgccag aagtccactt taacacttgt tgattacgac
      781 tttgtgacat gttgggagac aaatgttgat tgttttggcc aatggcaaag tttccaccgc
      841 ctaatatcat tcggggctgt acagccattg ttgcggtca
//
