LOCUS       sequence-154057-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila 
            cell culture. Please note- this plasmid does not express GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pAct:dCas9-VPR
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..34
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagatatcga caagtttgta caaaaaagca ggctacaaag ccaccatgga caagaagtac
       61 tccattgggc tcgctatcgg cacaaacagc gtcggctggg ccgtcattac ggacgagtac
      121 aaggtgccga gcaaaaaatt caaagttctg ggcaataccg atcgccacag cataaagaag
      181 aacctcattg gcgccctcct gttcgactcc ggggagacgg ccgaagccac gcggctcaaa
      241 agaacagcac ggcgcagata tacccgcaga aagaatcgga tctgctacct gcaggagatc
      301 tttagtaatg agatggctaa ggtggatgac tctttcttcc ataggctgga ggagtccttt
      361 ttggtggagg aggataaaaa gcacgagcgc cacccaatct ttggcaatat cgtggacgag
      421 gtggcgtacc atgaaaagta cccaaccata tatcatctga ggaagaagct tgtagacagt
      481 actgataagg ctgacttgcg gttgatctat ctcgcgctgg cgcatatgat caaatttcgg
      541 ggacacttcc tcatcgaggg ggacctgaac ccagacaaca gcgatgtcga taaactcttt
      601 atccaactgg ttcagactta caatcagctt ttcgaagaga acccgatcaa cgcatccgga
      661 gttgacgcca aagcaatcct gagcgctagg ctgtccaaat cccggcggct cgaaaacctc
      721 atcgcacagc tccctgggga gaagaagaac ggcctgtttg gtaatcttat cgccctgtca
      781 ctcgggctga cccccaactt taaatctaac ttcgacctgg ccgaagatgc ca
//
