LOCUS       sequence-154058-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila 
            cell culture. Please note- this plasmid does not express GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pAct:dCas9-VPR
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    31..55
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(60..629)
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
ORIGIN
        1 tcgcccttgc tcaccatggt ggcggagctc accactttgt acaagaaagc tgggtttcaa
       61 aacagagatg tgtcgaagat ggacagtcct gtgctgatat gcatggcatg caagaggcac
      121 tcgtcgttca ggaaggtatc cagaatctcg ttcaattccg gggtcagggg tgagtccagg
      181 ttcagatcct cggtcatgga ctcaagtgtg gttgtcagct catccagatg gccccttggg
      241 ggcggatggg aaaggtccat ttggccacag attgcagcct cttccttctg gggaatcaca
      301 gtatcggcca tctcccgaag ggctttgaca gcctggctcg tctcttcatc gggatcctcc
      361 aacaggtgac tggcctcggg agtcactgcg ggcgctggat ccagtggctg agggactggt
      421 gccggggtca gtgacccgac tggctcatgt actggaccgg ttggtgttgg tgcgaggctg
      481 gcggggagtg ggcggttggc ccatggactt cctggaggat gaaatggccg gattcgtttt
      541 ggctggcaca cctcgcggcc ctcaaacacg tcactaatag cggagccggc ctcaggcttc
      601 ggcaaaaaca tcccttccct ggaatcccga gatcccgatc cgctacccag cagtgcggag
      661 aagtccatgt cggcgataga gctgaagtcc tcgtcgccag acagcagtcc attaggcagg
      721 cctggtgctc ccagaggggc aggagctgga tcaggaggcc tctgagcgcc tgtcacgagc
      781 cgggtgatgg cctcggggta ttccatcagc atgggctcgg tggtgtgagg ggccacaggg
      841 atgccctggt tcagcagctg ctggaactcg ctgttgtcca cgctggccag gtcggtgaac
      901 acggcaggat cggtgctgtt tcccagc
//
