LOCUS       sequence-154059-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in 
            Drosophila cell culture.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pAct:dCas9-GCN4
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..57
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      106..115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agtttgtaca aaaaagcagg ctacaaagcc accatgccca agaagaagcg caaggtggga
       61 cgcgtctgca ggatatcaag cttgcggtac cgcgggcccg ggatcgccac catggacaag
      121 aagtacagca tcggcctggc catcggcacc aactctgtgg gctgggccgt gatcaccgac
      181 gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc
      241 aagaagaacc tgatcggcgc cctgctgttc gacagcggag aaacagccga ggccacccgg
      301 ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa
      361 gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag
      421 tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg
      481 gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg
      541 gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag
      601 ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag
      661 ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc
      721 agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa
      781 aa
//
