LOCUS       sequence-154063-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in 
            Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pAct:dCas9-VP64
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..84
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 agtttgtaca aaaaagcagg ctacaaagcc accatgaaaa ggccggcggc cacgaaaaag
       61 gccggccagg caaaaaagaa aaaggacaag aagtacagca tcggcctggc catcggcacc
      121 aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag
      181 gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggngc cctgctgttc
      241 gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc
      301 agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg
      361 gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac
      421 gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc
      481 accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg
      541 atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac
      601 ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac
      661 cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct
      721 gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag
      781 aagaatggcc tgttcggcaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag
      841 agcaactt
//
