LOCUS       sequence-154065-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MS2-p65-HSF under actin promoter for SAM CRISPRa in 
            Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pAct:MS2-p65-HSF
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             37..423
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             478..498
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agtttgtaca aaaaagcagg ctacaaagcc accatggctt caaactttac tcagttcgtg
       61 ctcgtggaca atggtgggac aggggatgtg acagtggctc cttctaattt cgctaatggg
      121 gtggcagagt ggatcagctc caactcacgg agccaggcct acaaggtgac atgcagcgtc
      181 aggcagtcta gtgcccagaa gagaaagtat accatcaagg tggaggtccc caaagtggct
      241 acccagacag tgggcggagt cgaactgcct gtcgccgctt ggaggtccta cctgaacatg
      301 gagctcacta tcccaatttt cgctaccaat tctgactgtg aactcatcgt gaaggcaatg
      361 caggggctcc tcaaagacgg taatcctatc ccttccgcca tcgccgctaa ctcaggtatc
      421 tacagcgctg gaggaggtgg aagcggagga ggaggaagcg gaggaggagg tagcggacct
      481 aagaaaaaga ggaaggtggc ggccgctgga tccccttcag ggcagatcag caaccaggcc
      541 ctggctctgg cccctagctc cgctccagtg ctggcccaga ctatggtgcc ctctagtgct
      601 atggtgcctc tggcccagcc acctgctcca gcccctgtgc tgaccccagg accaccccag
      661 tcactgagcg ctccagtgcc caagtctaca caggccggcg aggggactct gagtgaagct
      721 ctgctgcacc tgcagttcga cgctgatgag gacctgggag ctctgctggg gaacagcacc
      781 gatcccggag tgttcacaga tctggcctcc gtggacaact ctgagtttca gcagctgctg
      841 aatcagggcg tgtccatgtc tcatagtaca gccgaaccaa tgctgatgga
//
