LOCUS       sequence-154066-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MS2-p65-HSF under actin promoter for SAM CRISPRa in 
            Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pAct:MS2-p65-HSF
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    36..60
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcgg agctcaccac tttgtacaag aaagctgggt
       61 tttactgcag atcctttgcc ctaggacgag tgctggggcg tctgtttcca ctatcggcga
      121 gcacttccac acagccatcg gtccagacgg cagcgcttct gcgggcgatt tgtgtacgcc
      181 cgacagtccc ggctccggac cggacgattg cgtcgcagcg accctgagcc caagctgcat
      241 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
      301 aagcccggag ccgaggagat cctgcaagct cgggatgcct ccgctcgaag tagcgggtct
      361 gctgctccat acaagccaac cagggcctcc agaagaagat gttggcgacc tcgtattggg
      421 aatccccgaa catggcctcg ctccagtcaa tgacggctgt tatgcggcca ttgtcggtca
      481 ggacattgtt ggagccgaaa tcggcgtgca cgaggtgccg gacttcgggg cagtcctcgg
      541 cccaaagcat cagctcatcg agagcctggg cgacggaggc actgacggtg tcgtccatca
      601 cagtttgcca gtgatacaca tggggatcag caatagcgca gatgaaatcg cgccatgtag
      661 tgtattgacc gattccttga ggtccgaatg ggccgaaccc gctggtctgg ctaagatcgg
      721 cggcagcgat ggcatccatg gcctcggcga cgggctggag aacagcgggc agttcggttt
      781 caggcaggtc ttgcaaggtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
      841 cgctgaactc cccaatgtca agcacttcgg gaatagggag agcggcggat gcaaagtgcc
      901 gataaacata gcgatctttg tagaaaccat cggcgcagct attcacccgc
//
