LOCUS       sequence-154067-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9 under actin promoter for ""Scaffold"" CRISPRa in 
            Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pAct:dCas9
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..41
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccggatccag atatcgacaa gtttgtacaa aaaagcaggc tacaaagcca ccatggacaa
       61 gaagtactcc attgggctcg ctatcggcac aaacagcgtc ggctgggccg tcattacgga
      121 cgagtacaag gtgccgagca aaaaattcaa agttctgggc aataccgatc gccacagcat
      181 aaagaagaac ctcattggcg ccctcctgtt cgactccggg gagacggccg aagccacgcg
      241 gctcaaaaga acagcacggc gcagatatac ccgcagaaag aatcggatct gctacctgca
      301 ggagatcttt agtaatgaga tggctaaggt ggatgactct ttcttccata ggctggagga
      361 gtcctttttg gtggaggagg ataaaaagca cgagcgccac ccaatctttg gcaatatcgt
      421 ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat catctgagga agaagcttgt
      481 agacagtact gataaggctg acttgcggtt gatctatctc gcgctggcgc atatgatcaa
      541 atttcgggga cacttcctca tcgaggggga cctgaaccca gacaacagcg atgtcgataa
      601 actctttatc caactggttc agacttacaa tcagcttttc gaagagaacc cgatcaacgc
      661 atccggagtt gacgccaaag caatcctgag cgctaggctg tccaaatccc ggcggctcga
      721 aaacctcatc gcacagctcc ctggggagaa gaagaacggc ctgtttggta atcttatcgc
      781 cctgtcactc gggctgaccc ccaactttaa atctaacttc gacctggccg aagatgccaa
      841 gcttcaactg agcaaagaca cctacgatga tgatctcgac aatctgctgg
//
