LOCUS       sequence-154068-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9 under actin promoter for ""Scaffold"" CRISPRa in 
            Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pAct:dCas9
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    36..60
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(65..85)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcgg agctcaccac tttgtacaag aaagctgggt
       61 tttacacctt cctcttcttc ttggggtcag ccctgctgtc tccaccgagc tgagagaggt
      121 cgattcttgt ttcatagagc cccgtaattg actgatgaat cagtgtggcg tccaggacct
      181 cctttgtaga ggtgtaccgc tttctgtcta tggtggtgtc gaagtacttg aaggctgcag
      241 gcgcgcccaa gttggtcaga gtaaacaagt ggataatgtt ttctgcctgc tccctgatgg
      301 gcttatccct gtgcttattg taagcagaaa gcaccttatc gaggttagcg tcggcgagga
      361 tcactctttt ggagaattcg cttatttgct cgatgatctc atcaaggtag tgtttgtgtt
      421 gttccacgaa cagctgcttc tgctcattat cttcgggaga ccctttgagc ttttcatagt
      481 ggctggccag atacaagaaa ttaacgtatt tagagggcag tgccagctcg ttacctttct
      541 gcagctcgcc cgcactagcg agcattcgtt tccggccgtt ttcaagctca aagagagagt
      601 acttgggaag cttaatgatg aggtcttttt tgacctcttt atatcctttc gcctcgagaa
      661 agtcgatggg gtttttttcg aagcttgatc gctccatgat tgtgatgccc agcagttcct
      721 tgacgctttt gagtttttta gacttccctt tctccacttt ggccacaacc agtacactgt
      781 aagcgactgt aggagaatcg aatccgccgt atttcttggg gtcccaatct tttttgcgtg
      841 cgatcagctt gtcgctgttc cttttcggga ggatactttc cttggagaag cctccggtct
      901 gtacttcggt ctttttaacg atgttcacct gcggcatgga caggaccttc cggactgtcg
      961 cgaaatccct acccttgtcc cacacgattt ct
//
