LOCUS       sequence-154070-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MCP:VP64 under actin promoter for ""Scaffold"" CRISPRa in 
            Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pAct:MCP-VP64
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    36..60
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(77..226)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(608..631)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(641..661)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      664..673
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(679..703)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcgg agctcaccac tttgtacaag aaagctgggt
       61 ttcatctaga gttaatcagc atgtccaggt cgaaatcatc aagggcgtca ctgccgagca
      121 tgtcgaggtc aaagtcatca agggcatccg aaccaagcat gtcaaggtca aaatcatcga
      181 gggcgtcact tcccagcata tccagatcaa aatcgtccaa tgcgtcagcc cgtccggaac
      241 cgctggcctc gtagatgccg gagtttgctg cgattgctga gggaatcggg tttccatctt
      301 ttaggagacc ttgcattgcc ttaacaataa gctcgcagtc ggaattcgtg gcgaaaattg
      361 gaatggttag ttccatattt aagtacgaac gccaggcgcc tttaggcacc tcgactttga
      421 tggtgtattt gcgattctgc gcagagctct gacgaacgct acaggttact ttgtaagcct
      481 gtgaacgcga gttagagctg atccattcag cgatcccgtt agcgaagttg cttggggcga
      541 cagtcacgtc gccagttccg ccattgtcga cgagaacgaa ctgagtaaag ttagaagcca
      601 ttctagattt atcgtcgtca tccttgtagt cagcggccgc caccttcctc tttttcttag
      661 gtcccatggt ggctttgtag cctgcttttt tgtacaaact tgtcgatatc tggatccggg
      721 gtctctggat tagacgactg ctggctgatg gagcggcttt gtgtcgggag gagtatccac
      781 acagcacaag aactcaaacg gtagtgatat gaaaactggt cccgcagccc gttttataaa
      841 gcccgcttag cgctaaaacc ttatcagccg caatgctctc cgctctcaag tcgcgttcaa
      901 aacttttacc atatatgggt atgaattgtt cgccgattgg gttttcattt acggagaatt
      961 tcctccgcaa ctgggtgttc gtgcttgtgt gtgtcttgta tatcgccatg aa
//
