LOCUS       sequence-154076-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for expressing a single sgRNA with SAM MS2 tail, under U6:3 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pCFD3-MS2tail-SAM
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(314..332)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(384..402)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        complement(438..858)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
ORIGIN
        1 gttggttggc acaccacaaa tatactgttg ccgagcacaa ttgtctagaa tgcatacgca
       61 ttaagcgaac attaaaaaga tgtgaaaaca taactattat gtctaaataa acacacgtca
      121 gatgtatgta cgtcaacgga aaaccattgt ctatatatta caattactaa atacatacca
      181 aattgaatac atattgatga aaaataataa atactggcga aagcaaaaaa acgaaacatt
      241 tttattttat tgaacaactc tcaggctcca ggtaggcaaa aaagcaccga ctcggtgcca
      301 cttggccctg cagacatggg tgatcctcat gttggccaag ttgataacgg actagcctta
      361 ttttaacttg ctaggccctg cagacatggg tgatcctcat gttggcctag ctctaaaaca
      421 ggtcttctcg aagaccccga cgttaaattg aaaataggtc tatatatacg aactgagtct
      481 ggaaaaagaa gttgagaatt ataaaaagta gtgagcactg gcgccctctc tgcttggcga
      541 gctaaccttt tcgcctcttg gctgagtagg tggcgtttca ttctactctg taaaattaat
      601 gtagaattga aacactatgg tcaaaaaata cttaggggca taagttataa aacgtatgaa
      661 atgaattttt atcaacctgg gctattcaaa aattttcgaa ttattttatg tatttttttt
      721 aatcgttttt cattataggt taaaatacac tttaaaagga attctttcct gtaaaataaa
      781 tataaataaa tatgctttat tgacagaaaa tttgatgttt ttgtatttga gtaggagcaa
      841 tcacaggtga gcaaaaaaga attcagatct caattgatcg gctaaatggt atgggcaaga
      901 aaaggtatgc aatataataa tctttt
//
