LOCUS       sequence-154077-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for expressing a single sgRNA with SAM MS2 tail, under U6:3 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pCFD3-MS2tail-SAM
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttgttatgt tatatgtatt atatgtcaga cataaagaaa aggaacacat caaatgtgat
       61 aacaaagact aaacaagtaa ttttattaca ccaaaacgac aaaacagtag gcagaacaaa
      121 caacgcatag ccaaacattg acgaattgga taccctgccg attgtcagac acttttgttg
      181 atcagtttct tgcgaatggt ctcgtccagc ggtggaatcg cctcgcgggg aatcagaaaa
      241 gtggacagat tgaacagatc cagaaacacc ttgtaccgat cactgaaacc aaaaaaaaac
      301 aaagggagaa cagtttgagt tcattgatcc ccgatataat cacatctgcg atgatcacct
      361 gagagtggag cgcanatatt gatatccaga cgagccacca gtgcccaact gttgggatcc
      421 aatcatgcgt tgcaccatga tcacgtgatt gtctgcggcg ggaatagaaa gtatttggtt
      481 aggaaaacca gtcttaaaca taagatatat ttataaaaga gtatcaaaga atgcaatact
      541 tacatctcca cttggttatt aacgagtcga tgtccatgag cagggtgagc aactggtgtg
      601 gttggctgaa cctgggttca tccctataga aggtgatcat gatggctccc tgaagggcac
      661 gatggctaaa ccggcgatcc ccacgacgca ccagtgcatc gtgcactgcc ggatcaaaga
      721 tggagcgata cacctcgcgt cgcttctcaa tgtccatgag gcggtagttt ttcgccttct
      781 ccacgggctc ctccatggcg ctctgtacct gcgcctccag gaatcgatcg acgctctcct
      841 gaaacttggc ccagaagttg aagccactct cctccagtcc gggcgtcctc tccagccatc
      901 gctgcactag ctccagtagc gagggatctt tctccga
//
