LOCUS       sequence-154079-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for expressing a single sgRNA with Scaffold MS2 tail, under 
            U6:3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pCFD3-MS2tail-Scaffold
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(326..344)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
ORIGIN
        1 ggttggcaca ccacaaatat actgttgccg agcacaattg tctagaatgc atacgcatta
       61 agcgaacatt aaaaagatgt gaaaacataa ctattatgtc taaataaaca cacgtcagat
      121 gtatgtacgt caacggaaaa ccattgtcta tatattacaa ttactaaata cataccaaat
      181 tgaatacata ttgatgaaaa ataataaata ctggcgaaag caaaaaaacg aaacattttt
      241 attttattga acaactctca ggctccaggt aggcaaaaaa aaaaaacaaa aaaagggaag
      301 actccccagt gactgtggga gtcgcacatg ggtgatcctc atgtgctccc gcaccgactc
      361 ggtgccactt tttcaagttg ataacggact agccttattt aaacttgcta tgctgtttcc
      421 agcatagctc ttaaacaggt cttctcgaag accccgacgt taaattgaaa ataggtctat
      481 atatacgaac tgagtctgga aaaagaagtt gagaattata aaaagtagtg agcactggcg
      541 ccctctctgc ttggcgagct aaccttttcg cctcttggct gagtaggtgg cgtttcattc
      601 tactctgtaa aattaatgta gaattgaaac actatggtca aaaaatactt aggggcataa
      661 gttataaaac gtatgaaatg aatttttatc aacctgggct attcaaaaat tttcgaatta
      721 ttttatgtat tttttttaat cgtttttcat tataggttaa aatacacttt aaaaggaatt
      781 ctttcctgta aaataaatat aaa
//
