LOCUS       sequence-154080-         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for expressing a single sgRNA with Scaffold MS2 tail, under 
            U6:3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pCFD3-MS2tail-Scaffold
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttttgttatg ttatatgtat tatatgtcag acataaagaa aaggaacaca tcaaatgtga
       61 taacaaagac taaacaagta attttattac accaaaacga caaaacagta ggcagaacaa
      121 acaacgcata gccaaacatt gacgaattgg ataccctgcc gattgtcaga cacttttgtt
      181 gatcagtttc ttgcgaatgg tctcgtccag cggtggaatc gcctcgcggg gaatcagaaa
      241 agtggacaga ttgaacagat ccagaaacac cttgtaccga tcactgaaac caaaaaaaaa
      301 caaagggaga acagtttgag ttcattgatc cccgatataa tcacatctgc gatgatcacc
      361 tgagagtgga gcgcanatat tgatatccag acgagccacc agtgcccaac tgttgggatc
      421 caatcatgcg ttgcaccatg atcacgtgat tgtctgcggc gggaatagaa agtatttggt
      481 taggaaaacc agtcttaaac ataagatata tttataaaag agtatcaaag aatgcaatac
      541 ttacatctcc acttggttat taacgagtcg atgtccatga gcagggtgag caactggtgt
      601 ggttggctga acctgggttc atccctatag aaggtgatca tgatggctcc ctgaagggca
      661 cgatggctaa accggcgatc cccacgacgc accagtgcat cgtgcactgc cggatcaaag
      721 atggagcgat acacctcgcg tcgcttctca atgtccatga ggcggtagtt tttcgccttc
      781 tccacgggct cctccatggc gctctgtacc tgcgcctcca ggaatcgatc gacgctctcc
      841 tgaaacttgg cccagaagtt gaagccactc tcctccagtc cgggcgtcct ctccagccat
      901 cgctgcacta gctccagtag cgagggatct ttct
//
