LOCUS       sequence-154085-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying araC-PBAD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW112pBAD-hrpV
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..20
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(24..67)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    70..91
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga
       61 tgatttctgg aattcgcggc cgcttctaga gttatgacaa cttgacggct acatcattca
      121 ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata
      181 cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca
      241 tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta
      301 agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa
      361 catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact
      421 gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta atcgcttcca
      481 tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt
      541 ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt
      601 catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc cattcatgcc
      661 agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac
      721 gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt cggcaaacaa
      781 attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg agaatataac
      841 ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc tcaatcggcg
      901 ttaaacccgc cacca
//
