LOCUS       sequence-154092-         599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            eCFP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB444
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(50..174)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(230..251)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(491..510)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
       61 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
      121 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgataa
      181 cagcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      241 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      301 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      361 caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat
      421 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      481 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      541 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctgg
//
