LOCUS       sequence-154094-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying J116-30gfp-t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW203J116-gfp
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(62..96)
                     /label=BBa_J23116
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.16"
     misc_feature    97..118
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      121..164
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(168..186)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 actccagtga aaagttcttc tcctttacgc atctagtatt tctcctcttt aatctctagt
       61 agctagcata gtccctagga ctgagctagc tgtcaactct agaagcggcc gcgaattcca
      121 gaaatcatcc ttagcgaaag ctaaggattt tttttatctg aaattctgcc tcgtgatacg
      181 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcaccat
      241 ggaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
      301 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
      361 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
      421 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
      481 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
      541 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
      601 taacgtgagt tttcgttcca ctgagcgtca gaccccttaa taagatgatc ttcttgagat
      661 cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt
      721 ttttcgaagg ttctctgagc taccaactct ttgaaccgag gtaactggct tggaggagcg
      781 cagtcaccaa aa
//
