LOCUS       sequence-154104-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying J105-30gfp-t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW205J105-gfp
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             51..62
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(74..108)
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     misc_feature    109..130
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      133..176
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(180..198)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttac gcatctagta tttctcctct
       61 ttaatctcta gtagctagca tagtacctag gactgagcta gccgtaaact ctagaagcgg
      121 ccgcgaattc cagaaatcat ccttagcgaa agctaaggat tttttttatc tgaaattctg
      181 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      241 aggtggcacc atggaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      301 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      361 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      421 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      481 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      541 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
      601 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagacccctt aataagatga
      661 tcttcttgag atcgttttgg tctgcgcgta atctcttgct ctgaaaacga aaaaaccgcc
      721 ttgcagggcg gtttttcgaa ggttctctga gctaccaact ctttgaaccg aggtaactgg
      781 cttggaggag cgcagtcacc aaaacttgtc ctttcag
//
