LOCUS       sequence-154119-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying J106-30gfp-t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW206J106-gfp
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..20
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(24..67)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    70..91
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        92..126
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             138..149
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             156..869
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(186..214)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     809..829
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga
       61 tgatttctgg aattcgcggc cgcttctaga gtttacggct agctcagtcc taggtatagt
      121 gctagctact agagattaaa gaggagaaat actagatgcg taaaggagaa gaacttttca
      181 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
      241 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
      301 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc
      361 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc
      421 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
      481 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
      541 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
      601 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac
      661 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
      721 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
      781 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
      841 ttacacatgg catggatgaa ctatacaaat aataatacta gagcc
//
