LOCUS       sequence-154122-         479 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for N-terminal fusion with 
            eYFP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB442
FEATURES             Location/Qualifiers
     source          1..479
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             100..351
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     288..309
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    361..385
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
       61 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
      121 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
      181 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      241 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
      301 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggctgttatc
      361 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
      421 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtc
//
