LOCUS       sequence-154126-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying J101-rbs32-arsR-t-ParsR-30gfp-t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW101ParsR-gfp
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     186..203
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(227..284)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    285..305
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(312..339)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(355..426)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(489..509)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
       61 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
      121 cgggactcga gattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
      181 agcgcagcga gtcagtgagc gaggaagcct gcataacgcg aagtaatctt ttcggtttta
      241 aagaaaaagg gcagggtggt gacaccttgc ccttttttgc cggactgcag cggccgctac
      301 tagtatataa acgcagaaag gcccacccga aggtgagcca gtgtgactct agtagagagc
      361 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
      421 tatttgatgc ctggctctag tattattatt tgtatagttc atccatgcca tgtgtaatcc
      481 cagcagctgt tacaaactca agaaggacca tgtggtctct cttttcgttg ggatctttcg
      541 aaagggcaga ttgtgtggac aggtaatggt tgtctggtaa aaggacaggg ccatcgccaa
      601 ttggagtatt ttgttgataa tggtctgcta gttgaacgct tccatcttca atgttgtgtc
      661 taattttgaa gttaactttg attccattct tttgtttgtc tgccatgatg tatacattgt
      721 gtgagttata gttgtattcc aatttgtgtc caagaatgtt tccatcttct ttaaaatcaa
      781 taccttttaa ctcgattcta ttaacaaggg tatcaccttc aaacttgact tcagcacgtg
      841 tcttgtagtt cccgtcatct ttgaaaaata tagttctttc ctgtacataa cc
//
