LOCUS       sequence-154130-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying J101-rbs32-arsR-t-ParsR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW100ParsR
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     450..468
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(472..515)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    518..539
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        540..574
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
ORIGIN
        1 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
       61 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
      121 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
      181 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      241 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      301 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      361 cctgcaactt tatccgcctc catccagtct attccatggt gccacctgac gtctaagaaa
      421 ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat
      481 aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg cttctagagt
      541 ttacagctag ctcagtcctt ggtattatgc tagctcctag ggtcacacag gaaagtacca
      601 tatgtcattt ctgttaccca tccaattgtt caaaattctt gctgatgaaa cccgtctggg
      661 catcgtttta ctgctcagcg aactgggaga gttatgcgtc tgcgatctct gcactgctct
      721 cgaccagtcg cagcccaaga tctcccgcca cctggcattg ctgcgtgaaa gcgggctatt
      781 gctggaccgc aagcaaggta agtgggttca ttaccgctta tcaccgcata ttccagcatg
      841 ggcgg
//
