LOCUS       sequence-154131-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying J101-rbs32-arsR-t-ParsR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW100ParsR
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     186..203
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    209..229
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(346..373)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(389..460)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aaatatggta tcgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
       61 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
      121 cgggactcga gattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
      181 agcgcagcga gtcagtgagc gaggaagcct gcagcggccg ctactagtat tgcaggtagt
      241 gtctctcttc gaagcggata agtcaaaaac atatatgact taacgaatgt gtaagtgcag
      301 aggaaggtaa taggtgtgaa ttttgagttg gctctagtat ataaacgcag aaaggcccac
      361 ccgaaggtga gccagtgtga ctctagtaga gagcgttcac cgacaaacaa cagataaaac
      421 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggct ctagtacccg
      481 gataaaacac atctgaaaat tcatgtgtgt ttagctaaat ttttaactgc aaatgttctt
      541 actgtccccg gaacagtttt gtcgagccag gttgcggaca atcgcctgaa ccttttcctg
      601 ttcacatcgc caggcctcat caataatttt cgccgcccat gctggaatat gcggtgataa
      661 gcggtaatga acccacttac cttgcttgcg gtccagcaat agcccgcttt cacgcagcaa
      721 tgccaggtgg cgggagatct tgggctgcga ctggtcgaga gcagtgcaga gatcgcagac
      781 gcataactct cccagttcgc tgagcagtaa a
//
