LOCUS       sequence-154132-        3381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSB3K3 carrying J101-rbs32-arsR-t-ParsR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW100ParsR
FEATURES             Location/Qualifiers
     source          1..3381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..35
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     terminator      479..550
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      566..593
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    710..730
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(870..1685)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(870..1685)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1593..1612
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1677..1696
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(2280..2824)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2360..2379)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3292..3310
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3314..3357)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3360..3381
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tttacagcta gctcagtcct tggtattatg ctagctccta gggtcacaca ggaaagtacc
       61 atatgtcatt tctgttaccc atccaattgt tcaaaattct tgctgatgaa acccgtctgg
      121 gcatcgtttt actgctcagc gaactgggag agttatgcgt ctgcgatctc tgcactgctc
      181 tcgaccagtc gcagcccaag atctcccgcc acctggcatt gctgcgtgaa agcgggctat
      241 tgctggaccg caagcaaggt aagtgggttc attaccgctt atcaccgcat attccagcat
      301 gggcggcgaa aattattgat gaggcctggc gatgtgaaca ggaaaaggtt caggcgattg
      361 tccgcaacct ggctcgacaa aactgttccg gggacagtaa gaacatttgc agttaaaaat
      421 ttagctaaac acacatgaat tttcagatgt gttttatccg ggtactagag ccaggcatca
      481 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      541 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
      601 actagagcca actcaaaatt cacacctatt accttcctct gcacttacac attcgttaag
      661 tcatatatgt ttttgactta tccgcttcga agagagacac tacctgcaat actagtagcg
      721 gccgctgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      781 ggtatcagct cactcaaagg cggtaatctc gagtcccgtc aagtcagcgt aatgctctgc
      841 cagtgttaca accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac
      901 tgcaatttat tcatatcagg attatcgata ccatattttt gaaaaagccg tttctgtaat
      961 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1021 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1081 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     1141 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1201 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1261 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1321 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     1381 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     1441 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     1501 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     1561 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     1621 tcagcatcca tgttggaatt taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg
     1681 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1741 atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga
     1801 ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac
     1861 cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa caaagctctc
     1921 atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc ctggtatgag
     1981 tcagcaacac cttcttcacg aggcagacct cagcgctagc ggagtgtata ctggcttact
     2041 atgttggcac tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc
     2101 accggtgcgt cagcagaata tgtgatacag gatatattcc gcttcctcgc tcactgactc
     2161 gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg gcggagattt
     2221 cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc aaagccgttt
     2281 ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg
     2341 cgaaacccga caggactata aagataccag gcgtttcccc tggcggctcc ctcgtgcgct
     2401 ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc
     2461 attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac
     2521 gaaccccccg ttcagtccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2581 ccggaaagac atgcaaaagc accactggca gcagccactg gtaattgatt tagaggagtt
     2641 agtcttgaag tcatgcgccg gttaaggcta aactgaaagg acaagttttg gtgactgcgc
     2701 tcctccaagc cagttacctc ggttcaaaga gttggtagct cagagaacct tcgaaaaacc
     2761 gccctgcaag gcggtttttt cgttttcaga gcaagagatt acgcgcagac caaaacgatc
     2821 tcaagaagat catcttatta aggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2881 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2941 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3001 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3061 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3121 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3181 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattccatg
     3241 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     3301 atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg
     3361 aattcgcggc cgcttctaga g
//
