LOCUS       sequence-154134-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP. Figures 3 and 4. Strain 8. Promoter: ppTEF1, minimal 
            Promoter: mCYC. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP228
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    114..163
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
ORIGIN
        1 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
       61 ttggtcatga gatcagatct gagctcgttt ggccgtggcc gtgctcgtcc tcgtcggccg
      121 gcttgtcgac gacggcggtc tccgtcgtca ggatcatccg ggccacaagc ttgctgacag
      181 aagcctcaag aaaaaaaaaa ttcttcttcg actatgctgg aggcagagat gatcgagccg
      241 gtagttaact atatatagct aaattggttc catcacctcg agtcagcatc tggttacgta
      301 actctggcaa ccagtaacac gcttaaggtt tggaacaaca ctaaactacc ttgcggtact
      361 accattgaca ctacacatcc ttaattccaa tcctgtctgg cctccttcac cttttaacca
      421 tcttgcccat tccaactcgt gtcagattgc gtatcaagtg aaaaaaaaaa aattttaaaa
      481 tctttaaccc aatcaggtaa taactgtcgc ctcttttatc tgccgcactg catgaggtgt
      541 ccccttagtg ggaaagagta ctgagccaac cctggaggac agcaagggaa aaatacctac
      601 aacttgcttc ataatggtcg taaaaacaat ccttgtcgga tataagtgtt gtagactgtc
      661 ccttatcctc tgcgatgttc ttcctctcaa agtttgcgat ttctctctat cagaattgcc
      721 atcaagagac tcaggactaa tttcgcagtc ccacacgcac tcgtacatga ttggctga
//
