LOCUS       sequence-154135-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter: GAP, minimal Promoter: GAP6. Figure 4. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP261
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    54..72
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    75..93
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 aattctgtgt tttgatagtt gttcaattga ttgaaatagg gacaaataaa ttatctctat
       61 cactgatagg gagatctcta tcactgatag ggaaatttaa agtctttggg tcaggagaaa
      121 ccaaaattgg gaaaggtgtt cgccttttat attcgattct ggtggtttcc aataatctca
      181 tgacatgcgt ccgcccgcta ttattgccag cgacggcgcg cccatagggt atcctcactc
      241 cgattgatga agagaccctt ctggttccaa tcctcactcc gattgatgtg acgtcaatgg
      301 gtggagtata tcctcactcc gattgatgag tgctctcgat gacttcgata tcctcactcc
      361 gattgatgaa ctccgcccca ttgacgcaaa tcctcactcc gattgatggt ggaacagccc
      421 accctgtaca tcctcactcc gattgatgtg cggcctcgag aagcagctga atcctcactc
      481 cgattgatga ctggaccgtc accaattgga gatcctcact ccgattgatg gtggtaaact
      541 cgcaatatcc tcactccgat tgatgaattg atctgccgcg atgacttcta tatgatatgc
      601 tagctctcac ttaatcttct gtactctgaa gaggagtggg aaataccaag aaaaacatca
      661 aactcgaatg attttcccaa acccctacca caagatattc atcagctgcg agataggctg
      721 atcaggagca agctcgtac
//
