LOCUS       sequence-154140-        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of constitutively active chick E2F4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Morillo SM, Abanto EP, Roman MJ, Frade JM
  TITLE     Nerve growth factor-induced cell cycle reentry in newborn neurons is
            triggered by p38MAPK-dependent E2F4 phosphorylation.
  JOURNAL   Mol Cell Biol. 2012 Jul;32(14):2722-37. doi: 10.1128/MCB.00239-12. 
            Epub 2012 May 14.
  PUBMED    22586272
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00239-12"; journalName: "Mol Cell Biol"; date: 
            "2012-07"; volume: "32"; issue: "14"; pages: "2722-37"
COMMENT     Sequence Label: pcDNA6-E2F4CA-Myc
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..29)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(39..80)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(141..170)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gtttaaactc aatggtgatg gtgatgatga ccggtacgcg tagaatcgag accgaggaga
       61 gggttaggga taggcttacc ttcgaagggc cctctagact cgagcggccg ccactgtgct
      121 ggatatctgc agaattctca cagatcctct tcagagatga gtttctgctc gaggttaagg
      181 acaggcacat cgaagaggtc acagacgcct tcgctctcat ccaggttgta gatgtagtcg
      241 tgatctccag gaggagggga gaggcggagc aagggagcaa acacttcaga tgacatcagc
      301 tcctccagca gctcagagtt catacattct ctcgttggat caaacatctc tgacagctct
      361 ttgggaagct ccagcattcc tgcaggatca ggtttgattg gctcaaagga ggcagagggg
      421 ctgggtagga cggagctgct gtccagcgag gcagaggact gcagcagctg tgtgtccagc
      481 ctggctgtat ggccgctggg tgcagacaca ttgctgaggg ggtcaaggtc actgctgctg
      541 ctcttggact ccaccactga gatgctgcat tctgtgctcg tgtctttctg ctcaggaggg
      601 ctgtgatcct gagtgctgga aggttctggc tctgagggct gggtgctgct ggggacagat
      661 gcctcctgga agtgggcaag cggagggatc tgtggtttcg agggtgcaac tgcctggcac
      721 tggatgaggt cttcaggggg tgggacaggt agcactactg gaggagagct ccaagcatcc
      781 ttgtttacta agagaacatc aatcggaccg ctggtgctct tcagatggat ctgatatttc
      841 ttctgcctac ttgggccctc agggatggga acctctaggc gtgtgcctga aggggctcga
      901 attgcaagga gggtatctcc tgtgaagcac ttgcagatat cttcgtgtgt cacataagct
      961 agccagttgt tctgcacgtc ttctgtgaca tttttgatgc tctgctgaac ccacatcttc
     1021 tgcttctcca gctcctgctc ctgctgctcc aggtcttcta tatctgcctt cagctcaata
     1081 agcttgtgag caatc
//
