LOCUS       sequence-154142-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter: GAP, minimal Promoter: GAP. Figure 4. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP253
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    114..163
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
ORIGIN
        1 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
       61 ttggtcatga gatcagatct gagctcgttt ggccgtggcc gtgctcgtcc tcgtcggccg
      121 gcttgtcgac gacggcggtc tccgtcgtca ggatcatccg ggccacaagc ttgctgacag
      181 aagcctcaag aaaaaaaaaa ttcttcttcg actatgctgg aggcagagat gatcgagccg
      241 gtagttaact atatatagct aaattggttc catcacctcg agtcaattct tgatttagta
      301 tacacataac caaatttgga tcaagtttga agtaaaactt taacttcagc tccttacatt
      361 tgcactaaga tctctgctac tctggtccca agtgaaccac cttttggacc ctattgaccg
      421 gaccttaact tgccaaacct aaacgcttaa tgcctcagac gttttaatgc ctctcaacac
      481 ctccaaggtt gctttcttga gcatgcctac tagggacttt aacgaactgt ggggttgcag
      541 acagtttcag gcgtgtcccg accaatatgg cctactagac tctctgaaaa atcacagttt
      601 tccagtagtt ccgatcaaat taccatcgaa atggtcccat aaacggacat ttgacatccg
      661 ttcctgaatt atagtcttcc accgtggatc atggtgttcc tttttttccc aaagaatatc
      721 agcatccctt aactacgtta ggtcagtgat gacaatggac caaattgttg caaggttttt
      781 ctttttcttt catcgg
//
