LOCUS       sequence-154144-         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplemental Figure 4. Promoter GAP5. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP162
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    103..121
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    124..142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tgaattctgt gttttgatag ttgttcaatt gattgaaata gggacaaata aattaaattt
       61 aaagtctttg ggtcaggaga aaccaaaatt gggaaaggtg tttctctatc actgataggg
      121 agatctctat cactgatagg gacgcctttt atattcgatt ctggtggttt ccaataatct
      181 catgacatgc gtccgcccgc tattattgcc agcgacggcc gggacttttc catccctggg
      241 ctgctaggtc gggtacacga cctccgtttt acccgcaacg taatgctggg aagagcattg
      301 ctgcaagggg gccgtagaag aagctctcca gcagagtaaa atttcctagg gacggtaacg
      361 ggcggtggaa ggagagagaa gggaagagac gtttctggtt ccattactcc acattaaagt
      421 tttaccccgg agaattttac gttgccagca ggtcgttcgg agttgcaacg gagccagata
      481 tttcagagat ggctacctga ttggacgagg acaccaagac atttctacaa aaaaggatcc
      541 tgtcaagtga aaggggcaat tttaagattt caatcttgat ggatttcatt gatcgataat
      601 agtcgcatgt gaggctgaaa tgtgccgatg aaagaaaaag aaaaaccttg caacaatttg
      661 gtccattgtc atcactgacc taacgtagtt aagggatgct gatattcttt gggaa
//
