LOCUS       sequence-154147-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBW100ParsR carrying 30hrpR-30hrpS-t-hrpL-30gfp-t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW103ParsR-Amp30C
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..20
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(24..67)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    70..91
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        92..126
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     terminator      570..641
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      657..684
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga
       61 tgatttctgg aattcgcggc cgcttctaga gtttacagct agctcagtcc ttggtattat
      121 gctagctcct agggtcacac aggaaagtac catatgtcat ttctgttacc catccaattg
      181 ttcaaaattc ttgctgatga aacccgtctg ggcatcgttt tactgctcag cgaactggga
      241 gagttatgcg tctgcgatct ctgcactgct ctcgaccagt cgcagcccaa gatctcccgc
      301 cacctggcat tgctgcgtga aagcgggcta ttgctggacc gcaagcaagg taagtgggtt
      361 cattaccgct tatcaccgca tattccagca tgggcggcga aaattattga tgaggcctgg
      421 cgatgtgaac aggaaaaggt tcaggcgatt gtccgcaacc tggctcgaca aaactgttcc
      481 ggggacagta agaacatttg cagttaaaaa tttagctaaa cacacatgaa ttttcagatg
      541 tgttttatcc gggtactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      601 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      661 caccttcggg tgggcctttc tgcgtttata tactagagcc aactcaaaat tcacacctat
      721 taccttcctc tgcacttaca cattcgttaa gtcatatatg ttttt
//
