LOCUS       sequence-154149-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying J109-30hrpR-32hrpS-t-hrpL-30gfp-t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW211J109-Amp32C
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             49..60
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(294..321)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(337..408)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aattgggaca actccagtga aaagttcttc tcctttacgc atctagtatt tctcctcttt
       61 aatctctagt agtacaagcc ctatagcgat aaccatgcca gcttaaaaat agaaataact
      121 gattgattta taaagatatt ttaaaatgtg aaattctttg taatacaaaa ctttgcgcaa
      181 accgtttttt gccacaacag cttttggcac aaaaacatcg gtcagcttcg caagggaact
      241 gatccgggac cgtgacccac tcagcggaca taatccggcc tctagtatat aaacgcagaa
      301 aggcccaccc gaaggtgagc cagtgtgact ctagtagaga gcgttcaccg acaaacaaca
      361 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggttat
      421 tagatctgca attctttgat gcgtcgatag agcgtacggc gtggcacgtc cagttccagg
      481 cttaccgaat ccacacaatt gtcgtggcgc ttgagcgact cctgaatcag ggctttttcg
      541 atcactcgca attgcgattt gagcccacag gccaagtgct cttcgccctg cggctcggcg
      601 cccagcaagg ggaaacccag cacatggcgt ttggctgccg ccttgagctc acggatattg
      661 ccgggccagt cgtggcccag cagcactttg tgcagcagtg ggcaaacatc gggaacggga
      721 acaccgagct ccctcgcggc ggcggccgta aaacgtgtga acaggggaac tatgcgatca
      781 gactggttac gtagcggagg aagcttgagt gtcaggacgt tcaggcgaaa atacagatcg
      841 cgacgaaact gcccccgctc gacggcgtcg tccagcgagc attggg
//
